Regulatory
Part:BBa_K3831025
Designed by: Barbora Hrnčířová Group: iGEM21_Brno_Czech_Republic (2021-10-07)
Pgrac OcI promoter
The Pgrac promoter was created by combining the groE promoter, gsiBSD sequence and lacO operator to allow for IPTG induction. We took out the lacO sequence and instead of it we inserted the cI operator into the promoter sequence. This design should allow the strong promoter to constitutively express the gene downstream of it until the expression is stopped by the cI repressor originating from Phage lambda.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//promoter
chassis | Bacillus subtilis |